Speaking on the condition of anonymity before Centcom's announcement of fresh strikes, the US official insisted that US negotiators would continue to work in "good faith" towards a final deal with Iran.
Qatar's foreign ministry spokesperson Majed Al Ansari said it held Iran "fully responsible" for an apparent targeted attack on a vessel called Al-Rekayyat as it transited near the Strait.
Qatar demanded that Iran "immediately cease all practices that undermine regional security" and "refrain from endangering global energy supplies & the resources of the countries of the region in pursuit of narrow interests", he added in a post on X.
In a separate social media post, Saudi Arabia's foreign ministry said Iran had targeted the Saudi tanker Wadyan as it crossed the Strait.
It added that the assaults were "an attack on the security and safety of international navigation, and the security of global energy supplies".
Iran's foreign ministry spokesman Esmail Baghaei described Qatar's accusations, as "contrary to the principle of good neighbourliness".
In a statement, posted to Telegram, he added that commercial vessels using routes not coordinated with Iran or tampering with the ship's tracking face a risk of collision and disrupt Iran's efforts to "facilitate safe transit" in the Strait.
The UKMTO said a tanker travelling through the Strait had reported a fire after an unknown projectile hit an engine room on Monday.
In two separate incidents on Tuesday, a tanker reported being hit as it exited the Strait but was able to proceed to its next port of call, while another tanker reported sustaining minor structural damage after being struck, the organisation said.
The US-Iran memorandum of understanding , agreed last month, extended a ceasefire between the two countries.
The 14-point agreement would put an end to all conflict 'on all fronts', says that Iran will never have a nuclear weapon, and also commits a $300bn (£220bn) fund for the "reconstruction and economic development" of the country - although the US is not required to contribute.